News

The Final ImResFun Scientific Conference of the EC-funded Marie-Curie Initial Training Network ImResFun is now announced.

Publication: Candidalysin is a fungal peptide toxin critical for mucosal infection

ITN Practical Course "C2a", Barcelona, Spain (May 10-15, 2015)

ITN Meeting, La Colle sur Loup, France (May 16, 2015)

HPF2015, La Colle sur Loup, France (May 16-22, 2015)

ITN Practical Course "S3" & "C3", Madrid, Spain (July 6-10, 2015)

ITN Mid-Term Review Meeting, Vienna, Austria (November 3, 2015)

ITN Practical Course "S5" & "C5", Tübingen, Germany (May 9-11, 2016)

ITN Lecture Course "S4" & "C4", Szeged, Hungary (July 2-8, 2016)

ITN Practical Course "S6" & "C6", Göttingen, Germany (October 26-28, 2016)

ITN Symposium "S7" & Final Consortium Meeting, Innsbruck, Austria (January 28-30, 2017)

 

Name & Address

Steffen Rupp Portrait

Steffen RuppDepartment Head Molecular Biotechnology
Fraunhofer IGB
Nobelstr. 12, 70569 Stuttgart, Germany
Phone: +49-711-9704045 FAX: +49-711-9704200
e-mail:
Web: http://www.igb.fraunhofer.de/

ESR-FhG Project: (Start October/November 2013)

New assay systems for screening of antifungals / PRR-modulators.

The ESR will study interaction of fungal pathogen with the innate immune system. He/she will improve and adapt existing PRR-reporter cell lines, and develop screening assays for analyzing collections of chemical compounds and selected fungal PAMPS (pathogen associated molecular patterns) for PRR-modulating activity. In addition the ESR will use additional reporter assay and simple tissue models as drug-screening tools to serve as a basis for novel drug sceening assays.

ER-FhG Project: (Start January / February 2014)

Development of in vitro generated epithelial 3D-host-pathogen interaction systems

The ER will investigate fungal-host interaction using 3D-tissue models composed of cell lines and primary cells, including immune cells. A main goal is to study the cellular response to fungal pathogens and in paralle moitor fungal reaction. Both, host and fungal response will be analysed in parallel using RNA-Seq technologies. The results obtained will define novel biomarkers and drug targets.

Main Research Interests

Our research interests focus on host-pathogen interaction and the development of diagnostics and drug screening tools. We have developed several host-pathogen interaction models based on human cell lines and primary cells as well as screening assays for drug discovery and diagnostics based on microarray technologies. To analyze host-pathogen interaction in 3D-tissue models we use Next Generation Sequencing approaches and Proteomics methodologies. The main research interests focus on human fungal pathogens such as Candida spp. The aim is to improve our understanding of the molecular mechanisms underlying fungal virulence and commensalism. Especially interaction between the innate immune system and the fungal pathogen are in the focus of our interest, resulting in a special interest in cell wall biogenesis. To analyze this interaction we study simultaneously the mole-cular processes occuring both in the pathogen and the host. Both functional analyses of individual genes as well as comparative genomic approaches and genome-wide analyses of host-pathogen interaction on a genomic, transcriptomic and proteomic levels are methodologies used within the lab. This is backed up by systems biological approaches to identify the regulatory networks involved in host defense and fungal attack mechanisms. In addition high level expertise in cell culture technologies up to tissue engineering have been developed in the lab to set up host-pathogen interaction models. Using these technologies we aim to understand the molecular basis of fungal virulence and host immune response.

Selected Recent Publications

Grumaz, C., Lorenz, S., Stevens, P., Lindemann, E., Schöck, U., Retey, J., Rupp, S., Sohn, K. (2013) Species and condition specific adaptation of the transcriptional landscapes in Candida albicans and Candida dubliniensis. BMC Genomics. Apr 2;14(1):212.

Purschke FG, Hiller E, Trick I, Rupp S. (2012) Flexible survival strategies of Pseudomonas aeruginosa in biofilms result in increased fitness compared to Candida albicans. Mol Cell Proteomics. 11(12):1652-69

Zavrel, M., Maier, O., Kuchler, K. and Rupp S. (2012) The transcription factor Efg1 shows a haploinsufficiency phenotype in modulating cell wall architecture and immunogenicity of C. albicans Eukaryotic Cell, 11:129-40.

Burger-Kentischer, A., Finkelmeier, D., Bauer, J., Eickhoff, H., Kleymann, G., Rayyan, A., Schröppel, K., Wiesmüller, K.H. and Rupp, S. (2011) A screening assay based on host-pathogen interaction models identifies of a set of novel antifungal benzimidazole derivatives. Antimicrob. Agents Chemotherapy, 55(10):4789-801.

Burger-Kentischer, A., Abele, I.S., Finkelmeier, D., Wiesmüller, K.H. and Rupp, S. (2010) A new cell-based innate immune receptor assay for the examination of receptor activity, ligand specificity, signalling pathways and the detection of pyrogens. Journal of Immunological Methods, 358(1-2):93-103.