News

The Final ImResFun Scientific Conference of the EC-funded Marie-Curie Initial Training Network ImResFun is now announced.

Publication: Candidalysin is a fungal peptide toxin critical for mucosal infection

ITN Practical Course "C2a", Barcelona, Spain (May 10-15, 2015)

ITN Meeting, La Colle sur Loup, France (May 16, 2015)

HPF2015, La Colle sur Loup, France (May 16-22, 2015)

ITN Practical Course "S3" & "C3", Madrid, Spain (July 6-10, 2015)

ITN Mid-Term Review Meeting, Vienna, Austria (November 3, 2015)

ITN Practical Course "S5" & "C5", Tübingen, Germany (May 9-11, 2016)

ITN Lecture Course "S4" & "C4", Szeged, Hungary (July 2-8, 2016)

ITN Practical Course "S6" & "C6", Göttingen, Germany (October 26-28, 2016)

ITN Symposium "S7" & Final Consortium Meeting, Innsbruck, Austria (January 28-30, 2017)

 

Name & Address

Concha Gil Portrait

Concha GilProfessor of Microbiology
Department of Microbiology II and Proteomics Unit UCM-PCM
Complutense Unversity of Madrid
Plaza Ramón y Cajal s/n 28040 Madrid, Spain
Phone: +34-91-3941755 FAX: +34-91-3941745
e-mail:
Web: www.ucm.es/info/mfar & www.ucm.es/info/gyp/proteomica & http://dacya.ucm.es/proteopathogen

ESR-UCM Project: (Start January/ February 2014)

Immune-proteomics to identify novel biomarkers for invasive candidiasis

The ESR will study the immunome of Candida albicans to identify clinical biomarkers for invasive candidiasis and its phosphoproteome to understand signaling response pathways in immune cells using discovery and targeted proteomics and bioinformatic tools.

Main Research Interests

The main research lines in our group are related to proteomics of the Candida-host interaction to improve our knowledge on molecular mechanisms of host innate and acquired immune responses in candidiasis. Our objectives are to search novel biomarker and vaccine candidates for invasive candidiasis and to monitor the activation of signaling pathways in innate immune cells interacting with Candida cells. We use discovery proteomics, based on shotgun strategies, to acquire an overview of the proteome Candida cells upon interaction with its host. Then focusing on a selected set of proteins of interest, we use targeted proteomics, based on Selected Reaction Monitoring (SRM), which enables validation and quantitation for the target set with very high reproducibility and sensitivity. The combined use of both discovery and targeted approaches is therefore very useful to address this host-pathogen interaction model from the perspective of (clinical) proteomics. We have a great interest in the study of particular proteomes with great potential clinical applications. In this respect we study the phosphoproteomic profiles of immune activation in innate immune cells; the surfome, i.e. the set of proteins present in the fungal surface which first contact cells of the host immune system; and the set of proteins involved in apoptosis, since those would make an ideal target to selectively trigger this process. We are also involved in the Human Proteome Project (HPP) where Spain is responsible for developing SRM methods to detect proteins encoded in chromosome 16 (chromosome-centric HPP, or C-HPP) and try, at the same time to integrate the acquired methodology with the biology/disease branch of the HPP, (B/D-HPP) by focusing towards the proteins involved in the defense against candidiasis and mycoses in general.

Selected Recent Publications

Pitarch A, Nombela C, Gil C (2011). Prediction of the clinical outcome in invasive candidiasis patients based on molecular fingerprints of five anti-Candida antibodies serum. Mol Cell Proteomics 10(1):M110.004010. doi: 10.1074/mcp.M110.004010

Reales–Calderón JA, Sylvester M, Strijbis K, Jensen ON, Nombela C, Molero G, Gil C (2013 in press) Candida albicans induces pro-inflammatory and anti-apoptotic signals in macrophages as revealed by quantitative proteomics and phosphoproteomics J Proteomics Jul 4. doi:pii: S1874-3919(13)00372-2. 10.1016/j.jprot.2013.06.026

Vialas V, Sun Z, Loureiro y Penha CV, Carrascal M, Abián J, Monteoliva L, Deutsch EW, Aebersold R, Moritz RL, Gil C. (2013 in press) A Candida albicans PeptideAtlas. J Proteomics Jun 26. doi:pii: S1874-3919(13)00337-0. 10.1016/j.jprot.2013.06.020